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Image Search Results
Journal: Redox Biology
Article Title: A novel S -sulfhydrated human serum albumin preparation suppresses melanin synthesis
doi: 10.1016/j.redox.2017.10.007
Figure Lengend Snippet: Effect of Na 2 S n -treated HSA on melanin synthesis in B16 melanoma cells. Melanin content was measured by the absorption of at 405 nm after incubating Na 2 S n -treated HSA with 0.4 mM tyrosine and 10 mM NH 4 Cl for 72 h. Protein contents were analyzed by BCA protein Assay. Each value represents the mean ± S.E. n = 3. *p < 0.05 as compared with HSA. Cell image after the treatment with Na 2 S n -treated HSA in B16 melanoma cells. The photos were taken after a 72 h treatment with Na 2 S n -treated HSA in the presence of 0.4 mM tyrosine and 10 mM NH 4 Cl.
Article Snippet:
Techniques: Bicinchoninic Acid Protein Assay
Journal: Redox Biology
Article Title: A novel S -sulfhydrated human serum albumin preparation suppresses melanin synthesis
doi: 10.1016/j.redox.2017.10.007
Figure Lengend Snippet: ROS and NO scavenging effects of Na 2 S 4 -treated HSA under UV irradiation. ROS in B16 melanoma cells was detected by CM-H 2 DCF-DA in the presence of HSA and Na 2 S 4 -treated HSA with 15 min irradiation of 2 different UV, (A) 254 nm, (B) 365 nm. Each value represents the mean ± S.E. n = 3. **p < 0.01 as compared with control. ##p < 0.01 as compared with HSA. NO in B16 melanoma cells was detected by DAF-FM-DA in the presence of HSA and Na 2 S 4 -treated HSA after irradiation for 15 min with 2 different wavelengths, (C) 254 nm, (D) 365 nm. NO synthesis was measured by DAF-FM-DA. Each value represents the mean ± S.E. n = 3. *p < 0.05, **p < 0.01 as compared with control. ##p < 0.01 as compared with PBS.
Article Snippet:
Techniques: Irradiation, Control